Biolegend, 318340, Brilliant Violet 510™ anti-human CD56 (NCAM) Antibody, 100tests

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed Lima NS, et al. 2022. Nat Commun. 13:7733. PubMed Wang H, et al. 2022. Front Immunol. 13:852436. PubMed Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed Wang H, et al. 2022. iScience. 25:105065. PubMed Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed Tan J, et al. 2021. Sci Transl Med. 13: . PubMed Serra EG, et al. 2020. Nat Commun. 11:995. PubMed Abd Hamid M, et al. 2020. Cancer Immunology Research. 8(2):203-216. PubMed Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed Port JR, et al. 2020. J Virol. 94:. PubMed Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed Wang L, et al. 2021. Science. 373:. PubMed Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed Dacon C, et al. 2022. Cell Host Microbe. Online ahead of print. PubMed Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed Cho H, et al. 2021. Sci Transl Med. 13:eabj5413. PubMed Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
RRID: AB_2561385 (BioLegend Cat. No. 318339) AB_2561944 (BioLegend Cat. No. 318340)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: HCD56
Regulatory Status: RUO
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ