Biolegend, 318553, FITC anti-human CD45RA Antibody, 25tests

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD45RA transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone S23030B does not affect the binding of clone HI100 on target cells.
RRID: AB_3720536 (BioLegend Cat. No. 318553) AB_3720536 (BioLegend Cat. No. 318554)
Structure: Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution: B cells, naïve T cells, monocytes
Function: Involved in TCR and BCR signaling
Interaction: CD2, CD3, CD4
Ligand/Receptor: Galectin-1
Cell Type: B cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: Thomas M. 1989. Annu Rev Immunol. 7:339-69. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85-116.
Gene ID: 5788
UniProt: View information about CD45RA on UniProt.org
Clone: S23030B
Regulatory Status: RUO
Other Names: GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype: Mouse IgG1, κ