Biolegend, 318801, Purified anti-human CD270 (HVEM, TR2) Antibody, 25μg

The 122 antibody recognizes human HVEM also known as herpesvirus entry mediator A, tumor necrosis factor receptor superfamily, member 14, TNFRSF14, and tumor necrosis factor receptor like 2. HVEM, a member of the TNFR superfamily, is a type I transmembrane protein containing 2 TNF receptor domains with a predicted molecular weight of approximately 30 kD. HVEM is widely expressed in blood vessels, brain, heart, kidney, liver, lung, prostate, spleen, thymus and other organs. Resting T cells and naïve and memory B cells express high levels of HVEM as well. In humans, HVEM is not expressed in germinal center B cells. Immature dendritic cells express high levels of HVEM that is downregulated upon maturation. HVEM plays an important role in herpes simplex virus pathogenesis by enhancing entry into cells. Signaling through HVEM activates JNK1, NF-κB and AP-1 to control gene expression in response to infection or cellular stress and activate the immune response. HVEM binds to LIGHT and has also been shown to associate with several other proteins including TRAF1, TRAF2, TRAF3, TRAF5, B and T lymphocyte associated protein (BTLA), and estrogen receptor alpha.
25μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human HVEM protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 122 antibody has been shown to be useful for flow cytometry, Western blot, and ELISA.
Application References(PubMed link indicates BioLegend citation): Cheung TC, et al. 2010. J. Immunol. 185:1949. PubMed Hobo W, et al. 2012. J Immunol. 189:39. PubMed.
Product Citations: Malissen N, et al. 2019. Oncoimmunology. 8:e1665976. PubMed Cheung T, et al. 2010. J Immunol. 185:1949. PubMed Hobo W, et al. 2012. J Immunol. 189:39. PubMed
RRID: AB_2303380 (BioLegend Cat. No. 318802)
Structure: Member of the TNFR superfamily, type I transmembrane protein containing 2 TNF receptor domains. Predicted molecular weight approximately 30 kD.
Distribution: Widely expressed in blood vessels, brain, heart, kidney, liver, lung, prostate, spleen, thymus and other organs. Resting T cells and naïve and memory B cells express high levels of HVEM. Immature dendritic cells express high levels of HVEM that is downregulated upon maturation.
Function: Plays an important role in herpes simplex virus pathogenesis by enhancing entry into cells. Signaling through HVEM activates JNK1, NF-κB and AP-1 to control gene expression in response to infection or cellular stress and activate the immune response.
Interaction: TRAF1, TRAF2, TRAF3, TRAF5, B and T lymphocyte associated protein (BTLA), and estrogen receptor alpha have been shown to directly interact with HVEM in vivo.
Ligand/Receptor: LIGHT (TNFSF14), LTα
Cell Type: B cells, Dendritic cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Carfi A, et al. 2001. Molec. Cell 8:169. 2. Gonzalez LC, et al. 2005.Proc. Nat. Acad. Sci. 102:1116. 3. Kwon BS, et al. 1997. J. Biol. Chem. 272:13471. 4. Marsters SA, et al. 1997. J. Biol. Chem. 272:14272. 5. Montgomery RI, et al. 1996. Cell 87:427.
Gene ID: 8764
UniProt: View information about CD270 on UniProt.org
Clone: 122
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: TR2, Herpesvirus entry mediator A, Tumor necrosis factor receptor superfamily, member 14, TNFRSF14, Tumor necrosis factor receptor like 2, HVEM
Isotype: Mouse IgG1, κ