Biolegend, 319101, Anti-human CD3 FITC/(CD16+CD56) PE Cocktail, 50tests

CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes. CD16: 3G8 antibody reacts with CD16, a 50-65 kD transmembrane or a 48 kD GPI-anchored glycoprotein that is a member of the immunoglobulin superfamily. CD16 is expressed on NK cells, activated monocytes, macrophages, mast cells and neutrophils. CD56: MEM-188 antibody reacts with the 175 - 185 kD isoform of CD56, N-CAM (Neural Cell Adhesion Molecule), which is expressed on NK cells and a subset of CD3 + T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56.
50tests
Verified Reactivity: Human
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: This reagent is a combination of FITC conjugated UCHT1 and PE conjugated 3G8 and MEM-188 in optimal concentration for two-color flow cytometric analysis.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Note: Single color controls are required to perform instrument compensation and are not included in the kit.
Product Citations: Jeff Baker, Joshua Nederveen, Gianni Parise 2017. J Appl Physiol. 122(1):182-190. PubMed
RRID: AB_314999 (BioLegend Cat. No. 319101)
Distribution: T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes
Cell Type: Mesenchymal Stem Cells, NKT cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507. 4. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 5. Stroncek D, et al. 1991. Blood 77:1572. 6. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381. 7. Lanier L, et al. 1991. J. Immunol. 146:4421. 8. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 9. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 920
Clone: UCHT1; 3G8; MEM-188;
Regulatory Status: RUO
Isotype: Mouse IgG1/Mouse IgG2a
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.