Biolegend, 320002, Purified anti-mouse/rat/human FOXP3 Antibody, 100μg

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + /CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.
100μg
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: Cynomolgus, Rhesus, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length FOXP3 protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, the suggested working dilution(s) is ≤ 5.0 µg/ml in antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Application References(PubMed link indicates BioLegend citation): Roncador G, et al. 2005 Eur. J. Immunol. 35:1681. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol Yang ZZ, et al. 2006. Blood 107:3639. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. Groh V, et al. 2006. Nature Immunology 7:755. Lewkowicz P, et al. 2006 J. Immunol. 177:7155. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023. Bamias G, et al. 2007. J. Immunol. 178:1809. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed Davidson TS, et al. 2007. J. Immunol. 178:4022. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249. Jaffar Z, et al. 2007. J. Immunol. 179:6193. Müller M, et al. 2007. J. Immunol. 179:2774. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381 Klunker S, et al. 2009. J. Exp Med. PubMed Haque A, et al. 2010. J. Immunol. 184:2583. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
Product Citations: Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Berg S 2006. J Immunol. 176:2059. PubMed Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed Wang Y, et al. 2020. Ann Transl Med. 0.935416667. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed Miyabe C, et al. 2017. Ann Rheum Dis. 76:898. PubMed Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed Yang J, et al. 2019. Front Immunol. 2.492361111. PubMed Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
RRID: AB_439745 (BioLegend Cat. No. 320001) AB_439746 (BioLegend Cat. No. 320002)
Structure: Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution: Nuclear; expressed in T regulatory cells
Function: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction: Interacts with DNA
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hori S, et al. 2003. Science 299:1057.
Regulation: FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID: 2037131738250943
UniProt: View information about FOXP3 on UniProt.org
Clone: 150D
Regulatory Status: RUO
Other Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype: Mouse IgG1, κ
Q: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?
A: It is not recommended. It is best to use PBMCs for this testing.
Q: Can FOXP3 be costained with cytokines?
A: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.