Biolegend, 320007, PE anti-mouse/rat/human FOXP3 Antibody, 25tests

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + /CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.
25tests
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: Cynomolgus, Rhesus, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length FOXP3 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Roncador G, et al. 2005 Eur. J. Immunol. 35:1681. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol Yang ZZ, et al. 2006. Blood 107:3639. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. Groh V, et al. 2006. Nature Immunology 7:755. Lewkowicz P, et al. 2006 J. Immunol. 177:7155. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023. Bamias G, et al. 2007. J. Immunol. 178:1809. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed Davidson TS, et al. 2007. J. Immunol. 178:4022. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249. Jaffar Z, et al. 2007. J. Immunol. 179:6193. Müller M, et al. 2007. J. Immunol. 179:2774. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381 Klunker S, et al. 2009. J. Exp Med. PubMed Haque A, et al. 2010. J. Immunol. 184:2583. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
Product Citations: Barsheshet Y, et al. 2022. Int J Mol Sci. 23:. PubMed Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed Wu X, et al. 2023. iScience. 26:106559. PubMed Chamorro BM, et al. 2023. Vaccines (Basel). 11:. PubMed Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed Tian J, et al. 2021. Autophagy. 17:2586. PubMed Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed Mishra R, et al. 2022. J Transl Med. 20:323. PubMed Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed Zongyi Y, et al. 2017. PLoS One. 10.1371/journal.pone.0189617. PubMed Casadesús AV, et al. 2020. Oncoimmunology. 9:1770565. PubMed Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed Levi J, et al. 2020. J Nucl Med. . PubMed Deng Z, et al. 2020. World J Gastrointest Oncol. 1091:12. PubMed Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed Momin N, et al. 2022. Nat Commun. 13:109. PubMed Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed Tian X, et al. 2022. J Cell Mol Med. 26:693. PubMed Su X, et al. 2022. J Transl Med. 20:378. PubMed Fang J, et al. 2014. J Neuroimmunol. 269:9. PubMed Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed Wang R, et al. 2022. Front Immunol. 13:947756. PubMed Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed Yang Z, et al. 2021. Onco Targets Ther. 14:4239. PubMed Zeng Q, et al. 2022. iScience. 25:105151. PubMed Huang J, et al. 2014. J Immunol. 192:1972. PubMed Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed He S, et al. 2021. Front Pharmacol. 12:743837. PubMed Wang S, et al. 2022. J Inflamm Res. 14:7107. PubMed Tanaka S, et al. 2016. Appl Envir Microbiol. 82: 2693 - 2699. PubMed Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed Chen KH, et al. 2019. J Neuropathol Exp Neurol. 78:65. PubMed Jia Y, et al. 2022. J Transl Med. 20:281. PubMed Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed Rana MN, et al. 2021. Front Pharmacol. 12:643215. PubMed
RRID: AB_492981 (BioLegend Cat. No. 320007) AB_492980 (BioLegend Cat. No. 320008)
Structure: Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution: Nuclear; expressed in T regulatory cells
Function: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction: Interacts with DNA
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hori S, et al. 2003. Science 299:1057.
Regulation: FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID: 2037131738250943
UniProt: View information about FOXP3 on UniProt.org
Clone: 150D
Regulatory Status: RUO
Other Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?
A: It is not recommended. It is best to use PBMCs for this testing.
Q: Can FOXP3 be costained with cytokines?
A: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.