Biolegend, 320111, Alexa Fluor® 488 anti-human FOXP3 Antibody, 25tests

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + /CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.
25tests
Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus, Rhesus, Pigtailed Macaque
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length FOXP3 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1,8,19-20, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding. NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Application References(PubMed link indicates BioLegend citation): Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.(IHC) Yang ZZ, et al. 2006. Blood 107:3639. Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed Bollyky PL, et al. 2007. J. Immunol. 179:744. Bell MP, et al. 2007. J. Immunol. 179:1893. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656. PubMed Gao Q,et al.2007.J Clin Oncol.25:2586.(IHC) PubMed Pillai V,et al. 2008. Blood 111:463. PubMed Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed Zonios DI, et al. 2008.Blood112:287. PubMed Kavanagh B, et al. 2008. Blood. PubMed Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed Hodi FS, et al. 2014. Cancer Immunol Res. 2:632.(IHC) PubMed Sziros E, et al. 2015. Clin Cancer Res. 21:2840.(IHC) PubMed
Product Citations: Nettenstrom L, et al. 2013. J Immunol Methods. 387:81. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Pernaa N, et al. 2022. Front Immunol. 13:819929. PubMed Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed Nigam P, et al. 2010. J Immunol. 184:1690. PubMed Husseiny MI, et al. 2020. Front Genet. 11:300. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Wang S, et al. 2021. Exp Ther Med. 21:37. PubMed Kelsen J, et al. 2012. Cytokine. 59:403. PubMed Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed Zheng Y, et al. 2008. J Immunol. 181:1683. PubMed Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed Van Gool F, et al. 2019. Immunity. 50:362. PubMed Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed Kubin ME, et al. 2017. Acta Derm Venereol. 97:449. PubMed Liu W, et al. 2006. J Exp Med. 203:1701. PubMed Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed Riese P, et al. 2022. Nat Commun. 13:6894. PubMed Ryba M, et al. 2011. Cytokine. 55:353. PubMed Jin J, et al. 2014. PLoS One. 9:104753. PubMed Nevala W, et al. 2009. Clin Cancer Res. 1.965972222. PubMed
RRID: AB_430882 (BioLegend Cat. No. 320111) AB_430883 (BioLegend Cat. No. 320112)
Structure: Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution: Nuclear; expressed in T regulatory cells
Function: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction: Interacts with DNA
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hori S, et al. 2003. Science 299:1057. 2. Gandhi R, et al. 2010. Nat. Immunol. 11:846.
Regulation: FOXP3 is present at high levels in T regulatory cells, it can also be induced by T cell activation.
Gene ID: 50943
UniProt: View information about FOXP3 on UniProt.org
Clone: 206D
Regulatory Status: RUO
Other Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype: Mouse IgG1, κ
Q: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?
A: It is not recommended. It is best to use PBMCs for this testing.
Q: Can FOXP3 be costained with cytokines?
A: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.