Biolegend, 320608, PE anti-human CD181 (CXCR1) Antibody, 100tests

CXCR1 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor A (IL-8RA), CD181, and CDw128a. It is a CXC chemokine receptor that belongs to the G protein-coupled receptor (CPCR) family. CXCR1 is expressed as homodimer or heterodimer with CXCR2 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. The expression level of CXCR1 on monocytes is lower than that of CXCR2. CXCR1 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α(CXCL1).
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR1 transfected L1.2 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: The 8F1/CXCR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR1 expression.
Product Citations: Silvin A, et al. 2020. Cell. 182:1401. PubMed Brandau S, et al. 2011. J Leukoc Biol. 89:311. PubMed Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed Shin M, et al. 2015. J Immunol. 195: 2861-2869. PubMed
RRID: AB_439815 (BioLegend Cat. No. 320608)
Structure: Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR2
Distribution: Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes
Function: neutrophil activation, chemotaxis, angiogenesis
Ligand/Receptor: IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-a(CXCL1)
Cell Type: Endothelial cells, Granulocytes, Monocytes, Neutrophils, NK cells, Oligodendrocytes, T cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682. 2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663. 3. Emadi S, et al. 2005. Blood 105:464. 4. Omari KM, et al. 2005. Brain 128:1003. 5. Juremalm M, G. Nilsson. 2005. Chem. Immunol. Allergy. 87:130. 6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164
Gene ID: 3577
UniProt: View information about CD181 on UniProt.org
Clone: 8F1/CXCR1
Regulatory Status: RUO
Other Names: CD181, CXCR1, CDw128, CD128a, IL-8RA, Type I IL-8 receptor
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.