Biolegend, 320715, PE/Cyanine7 anti-human CD182 (CXCR2) Antibody, 25tests

CXCR2 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor B (IL-8RB), CD182, and CD128b. It is a CXC chemokine receptor belongs to G protein-coupled receptor (GPCR) family. CXCR2 is expressed as homodimer or heterodimer with CXCR1 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR2 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α,β,γ (CXCL1, CXCL2, CXCL3).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR2 transfected L1.2 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: The 5E8/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression.
Application References(PubMed link indicates BioLegend citation): Kyriakakis E, et al. 2011. J Leukoc Biol. 90:929. PubMed.
RRID: AB_2564596 (BioLegend Cat. No. 320715) AB_2564597 (BioLegend Cat. No. 320716)
Structure: Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1
Distribution: Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes
Function: Neutrophil activation, chemotaxis, angiogenesis
Ligand/Receptor: IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-& alpha;,ß, γ (CXCL1, CXCL2, CXCL3)
Cell Type: Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682. 2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663. 3. Emadi S, et al. 2005. Blood 105:464. 4. Omari KM, et al. 2005. Brain 128:1003. 5. Juremalm M and Nilsson G. 2005. Chem. Immunol. Allergy 87:130. 6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164.
Gene ID: 3579
UniProt: View information about CD182 on UniProt.org
Clone: 5E8/CXCR2
Regulatory Status: RUO
Other Names: CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor
Isotype: Mouse IgG1, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.