Biolegend, 320807, APC anti-human CD314 (NKG2D) Antibody, 25tests

CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8 + T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Application References(PubMed link indicates BioLegend citation): Wu J, et al. 1999. Science 285:730. Wu J, et al. 2000. J. Exp. Med. 192:1059. Groh V, et al. 2001. Nature Immunol. 2:255. Wu J, et al. 2002. J. Immunol. 169:1236. Roberts A, et al. 2001. J. Immunol. 167:5527. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Watanbe M, et al. 2014. Int Immunol. PubMed
Product Citations: Stitzlein LM, et al. 2023. Front Neurol. 14:1112207. PubMed Wang H, et al. 2022. Front Immunol. 13:852436. PubMed Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed Chen M, et al. 2021. Cancers (Basel). 13:. PubMed Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed Watanabe M, et al. 2014. Int Immunol. 26:597. PubMed de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed Welsh JA, et al. 2022. Cell Rep Methods. 2:100136. PubMed Wu X, et al. 2020. Cancers (Basel). 12:. PubMed Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed Paidipally P, et al. 2009. J Immunol. 183:1940. PubMed Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Bermudez-Brito M, Faas M, Vos P 2016. Hum Reprod. 31: 1462 - 1474. PubMed Wu X, et al. 2021. Front Immunol. 12:731767. PubMed Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed Oyer JL, et al. 2022. Front Immunol. 13:861681. PubMed Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
RRID: AB_492963 (BioLegend Cat. No. 320807) AB_492962 (BioLegend Cat. No. 320808)
Structure: C-type lectin
Distribution: NK cells, γ/δ T cells, CD8+ T cells
Function: Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor: MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type: NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Jamieson AM, et al. 2002. Immunity 17:19. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667. 7. Maasho K, et al. 2005. J. Immunol. 174:4480. 8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
Gene ID: 22914
UniProt: View information about CD314 on UniProt.org
Clone: 1D11
Regulatory Status: RUO
Other Names: NKG2D
Isotype: Mouse IgG1, κ