Biolegend, 320813, Ultra-LEAF™ Purified anti-human CD314 (NKG2D) Antibody, 100μg

CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8 + T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality testedIP, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Application References(PubMed link indicates BioLegend citation): Wu J, et al. 1999. Science 285:730. Wu J, et al. 2000. J. Exp. Med. 192:1059. Groh V, et al. 2001. Nature Immunol. 2:255. Wu J, et al. 2002. J. Immunol. 169:1236. Roberts A, et al. 2001. J. Immunol. 167:5527. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Watanbe M, et al. 2014. Int Immunol. PubMed
Product Citations: Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed Breman E, et al. 2019. Front Immunol. 9:2940. PubMed Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed Dudek M, et al. 2021. Nature. 592:444. PubMed Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed Glasner A, et al. 2017. Sci Rep. 7:40944. PubMed
RRID: AB_2810480 (BioLegend Cat. No. 320813) AB_2561488 (BioLegend Cat. No. 320814)
Structure: C-type lectin
Distribution: NK cells, γ/δ T cells, CD8+ T cells
Function: Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor: MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type: NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Jamieson AM, et al. 2002. Immunity 17:19. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667. 7. Maasho K, et al. 2005. J. Immunol. 174:4480. 8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
Gene ID: 22914
UniProt: View information about CD314 on UniProt.org
Clone: 1D11
Regulatory Status: RUO
Other Names: NKG2D
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.