Biolegend, 320902, Purified anti-human MICA/MICB Antibody, 100μg

6D4 antibody reacts with a common epitope of the human nonclassical MHC class I chain-related protein A (MICA) and B (MICB), also known as PERB11.1 and PERB11.2. The MIC gene is located in MHC class I region. MICA/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate β2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN-α-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8 + α/β T cells and γ/δ T cells, as well as suppression of T cell proliferation. MICA/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases. The 6D4 antibody is able to block MIC mediated cytotoxicity.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-P, IHC-F, IP, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported (for the relevant formats) applications include: immunohistochemistry2,3,5 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation7, and blocking2,3 of MIC mediated cytotoxicity. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 320919 & 320120).
Application References(PubMed link indicates BioLegend citation): Groh V, et al. 1999. Science 279:1737. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. Groh V, et al. 2001. Nature Immunol. 2:255. Li Z, et al. 2000. Immunogenetics 51:246. Park EJ, et al. 2003. J. Immunol. 171:4131. Jinushi M, et al. 2003. J. Immunol. 171:5423. Wu J, et al. 2003. J. Immunol. 170:4196.
Product Citations: Nakajima NI, et al. 2017. Oncol Rep. 38:693. PubMed Nolan E, et al. 2017. Sci Transl Med. 9:eaal4925. PubMed Tjwa E, et al. 2012. J Virol. 86:4102. PubMed Wang J, et al. 2022. Oncoimmunology. 11:2016159. PubMed Takahara M, et al. 2008. J Leukoc Biol. 83:742. PubMed
RRID: AB_493189 (BioLegend Cat. No. 320902)
Structure: 65-75 kD glycoprotein, MHC class I-like transmembrane molecules that do not associate ß2-microglobulin, highly polymorphic
Distribution: Intestinal epithelium, epithelial tumors, endothelial cells, fibroblasts, INF-a-stimulated dendritic cells
Function: Activation of NK cells, subset of CD8+ α/ß T cells and γ/δ T cells, suppression of T cell proliferation
Ligand/Receptor: NKG2D
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Groh V, et al. 1996. Proc. Natl. Acad. Sci. USA. 93:12445. 2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. 3. Jinushi M, et al. 2003. J. Immunol. 170:1249. 4. Kriegeskorte AK, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:11805. 5. Groh V, et al. 1999. Science 279:1737.
Gene ID: 1005074364277
UniProt: View information about MICA MICB on UniProt.org
Clone: 6D4
Regulatory Status: RUO
Other Names: PERB11, MIC, MHC class I chain-related protein A, MICA, PERB11.1, MHC class I chain-related protein B, MICB, PERB11.2
Isotype: Mouse IgG2a, κ