Biolegend, 321105, PE anti-human CD206 (MMR) Antibody, 25tests

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human mannose receptor
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).
Application References(PubMed link indicates BioLegend citation): Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block) Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block) Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations: Hao D, et al. 2022. Front Pharmacol. 13:1007623. PubMed Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed Shen WY, et al. 2023. iScience. 26:106805. PubMed Sefik E, et al. 2022. Nature. 606:585. PubMed Taffoni C 2022. The EMBO Journal. e111961. PubMed Huang L, et al. 2023. MedComm (2020). 4:e191. PubMed García-García A, et al. 2023. Bioact Mater. 24:174. PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Shen Y, et al. 2021. Cell Stress Chaperones. 26:937. PubMed Zhan X, et al. 2014. Biomaterials. 35:10046. PubMed Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Zhou WH, et al. 2022. Discov Oncol. 13:67. PubMed Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed Dan H, et al. 2020. Mol Oncol. 14:795. PubMed Jaén RI, et al. 2022. Int J Mol Sci. 23:. PubMed Rao X, et al. 2022. Cell Death Dis. 13:891. PubMed Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed Malekghasemi S, et al. 2020. Cell Biol Int. 44:2031. PubMed Yang M, et al. 2018. Oncol Lett. 15:3918. PubMed Zangeneh Z, et al. 2022. Iran J Basic Med Sci. 25:474. PubMed Sefik E, et al. 2021. Nat Biotechnol. . PubMed Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed Qin XH, et al. 2019. Langmuir. 35:1882. PubMed Lv J, et al. 2021. Mol Med Rep. 1. PubMed Cho JH, et al. 2021. Nat Commun. 12:792. PubMed Madsen NH, et al. 2021. Pathogens. 10:. PubMed Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed Kleinnijenhuis J, et al. 2012. Proc Natl Acad Sci U S A. 109:17537. PubMed Wilcz-Villega E, et al. 2020. EMBO Mol Med. 12:e10491. PubMed Georgouli M et al. 2019. Cell. 176(4):757-774 . PubMed Chen X, et al. 2022. Cell Commun Signal. 20:92. PubMed
RRID: AB_571910 (BioLegend Cat. No. 321105) AB_571911 (BioLegend Cat. No. 321106)
Structure: Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution: Macrophages, dendritic cells, hepatic and lymphatic endothelial cells
Function: Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor: Mannose, fucose, N-acetyl glucosamine
Cell Type: Dendritic cells, Endothelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303 2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469. 4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934. 5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.
Gene ID: 4360
UniProt: View information about CD206 on UniProt.org
Clone: 15-2
Regulatory Status: RUO
Other Names: MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.