Biolegend, 321122, PerCP/Cyanine5.5 anti-human CD206 (MMR) Antibody, 100tests

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human mannose receptor
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).
Application References(PubMed link indicates BioLegend citation): Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block) Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block) Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations: Douthwaite H, et al. 2022. Bio Protoc. 12:e4361. PubMed Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed Li C, et al. 2015. PLoS One. 10: e0141817. PubMed Ray SC, et al. 2018. J Immunol. 200:3568. PubMed Wu X, et al. 2020. Front Immunol. 1.922222222. PubMed Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed Vierboom MPM, et al. 2021. Cell Reports Medicine. 2(1):100185. PubMed
RRID: AB_10900990 (BioLegend Cat. No. 321121) AB_10899411 (BioLegend Cat. No. 321122)
Structure: Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution: Macrophages, dendritic cells, hepatic and lymphatic endothelial cells
Function: Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor: Mannose, fucose, N-acetyl glucosamine
Cell Type: Dendritic cells, Endothelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303 2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469. 4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934. 5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.
Gene ID: 4360
UniProt: View information about CD206 on UniProt.org
Clone: 15-2
Regulatory Status: RUO
Other Names: MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.