Biolegend, 321214, FITC anti-human/mouse Integrin β7 Antibody, 500μg

Integrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,α IEL /β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.
500μg
Verified Reactivity: Human, Mouse, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Chimpanzee
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TK1 cells
Formulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg sizes, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).
Application References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block) Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block) Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Röhl M, et al. 2021. Vaccines (Basel). 9:. PubMed Chu C, et al. 2013. PLoS One. 8:64187. PubMed Sato T, et al. 2020. Sci Rep. 10:13221. PubMed Ohmori K, et al. 2009. J Immunol. 182:2835. PubMed Durant L, et al. 2020. Microbiome. 8:88. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
RRID: AB_830857 (BioLegend Cat. No. 321213) AB_830856 (BioLegend Cat. No. 321212) AB_830858 (BioLegend Cat. No. 321214)
Structure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution: CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes
Function: Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand/Receptor: CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Picarella D, et al. 1997. J. Immunol. 158:2099. 3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635 4. Cepek KL, et al. 1994. Nautre 372:190.
Gene ID: 164213695
UniProt: View information about Integrin beta7 on UniProt.org
Clone: FIB504
Regulatory Status: RUO
Workshop: VI 6T-101, VI A024
Other Names: β7 Integrin, integrin βp, ITGB7
Isotype: Rat IgG2a, κ