Biolegend, 321606, PE anti-human CD195 (CCR5) Phospho (Ser349) Antibody, 100tests

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Human
Immunogen: Phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) of CCR5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The CD195 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The E11/19 monoclonal antibody binds to phosphoserine 349 corresponding to 22 c-terminal amino acid residues of human CCR5 and is useful for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1, and ELISA detection1 when paired with T21/8 as the capture antibody (Cat. No. 321401/321402).
Application References(PubMed link indicates BioLegend citation): Pollok-Kopp B, et al. 2003. J. Biol. Chem. 278:2190. (IF ELISA) Fox JM, et al. 2011. 117:1851. PubMed Scalley-Kim ML, et al. 2012. PLoS One. 7:e43332. PubMed.
Product Citations: Dahl M, et al. 2008. J Pharmacol Exp Ther. 327:926. PubMed Scalley-Kim M, et al. 2012. PLoS One. 7:e43332. PubMed Huffman A,et al. 2017. Biol Blood Marrow Transplant. . 10.1016/j.bbmt.2017.10.028. PubMed
RRID: AB_528973 (BioLegend Cat. No. 321606)
Structure: G-coupled receptor family 1, membrane protein, 45 kD
Distribution: Subset of T cells, monocytes
Function: Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1.
Ligand/Receptor: MIP-1a, MIP-1ß, RANTES
Cell Type: Monocytes, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR, Phospho-Proteins
Antigen References: 1. Samson M, et al. 1996. Biochemistry 35:3362. 2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161. 3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147. 4. Deng H, et al. 1996. Nature 381:661.
Gene ID: 1234
UniProt: View information about CD195 Phospho Ser349 on UniProt.org
Clone: E11/19
Regulatory Status: RUO
Other Names: CD195, CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.