Biolegend, 322008, PE anti-human Lymphotoxin beta receptor (LTβR) Antibody, 100tests

LT-βR, also known as lymphotoxin beta receptor and tumor necrosis factor receptor superfamily member 3 is a 47 kD transmembrane protein containing 3 TNF receptor domains. The LT-βR is a receptor for the heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT). LT-βR is highly expressed in the lung, liver, and kidney and moderately expressed expressed in the heart and testes. LT-βR is weakly expressed in the brain, thymus, spleen, and lymph nodes. The cytoplasmic tail of LT-βR interacts with TRAF3, TRAF4, and TRAF5 as well as the hepatitis C virus core protein. Ligand binding to LT-βR has been shown to induce apoptosis (via TRAF3 and TRAF5) and play a role in the development of lymphoid organs. The 31G4D8 antibody has been shown to be useful for flow cytometry, Western blot, immunoprepicitation and and ELISA (detection antibody). In addition, the 31G4D8 antibody is an agonistic antibody for the LT-βR capable of inducing apoptosis (with IFN-γ treatment).
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human LT-βR Fc protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, ELISA detection1, induction of apoptosis2. The biotinylated 31G4D8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified 36G9G3 antibody (Cat. No. 322101/322102) as capture antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 322003/322004).
Application References(PubMed link indicates BioLegend citation): Developer's communication Chen M-C, et al. 2003. J. Biol. Chem. 278:16073.
Product Citations: Rohwedder I, et al. 2023. JCI Insight. 8: . PubMed
RRID: AB_2139070 (BioLegend Cat. No. 322008)
Structure: Member of the TNF receptor superfamily; transmembrane receptor containing 3 TNF receptor domains, predicted molecular weight approximately 47 kD
Distribution: Highly expressed in lung, liver, and kidney. Moderate expression in heart and testes; weak expression in brain, thymus, spleen, and lymph nodes.
Function: Promotes apoptosis via TRAF3 and TRAF5, plays a role in the development of lymphoid organs
Interaction: Receptor for heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT. Also interacts with TRAF3, TRAF4, and TRAF5 as well as hepatitis C virus core protein
Cell Type: Embryonic Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Crowe PD, et al. 1994 Science 264:707.
Gene ID: 4055
UniProt: View information about LTbetaR on UniProt.org
Clone: 31G4D8
Regulatory Status: RUO
Other Names: Lymphotoxin beta receptor, Tumor necrosis factor receptor superfamily member 3
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.