Biolegend, 322808, APC anti-human TSLPR (TSLP-R) Antibody, 100tests

TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind with TSLP. The TSLP-R contains a WSXWS motif required for proper protein folding and a box1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight approximately 40 kD, and has two isoforms reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells, dendritic cells, and activated monocytes. It is weakly expressed in T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergy diseases.
100tests
Verified Reactivity: Human
Reported Reactivity: Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TSLP-R:Fc protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 322805/322806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)).
Product Citations: Alkashgari HR, et al. 2022. Int J Mol Sci. 24: . PubMed Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Scheeren F, et al. 2009. Eur J Immunol. 40:955. PubMed Sugimoto H, et al. 2010. Clin Immunol. 136:205. PubMed Gu C, et al. 2021. Front Immunol. 12:678036. PubMed Hulse K, et al. 2010. J Allergy Clin Immunol. 125:247. PubMed Qin H, et al. 2015. Blood. 126: 629 - 639. PubMed Brulefert A, et al. 2021. Allergy. 76:2044. PubMed
RRID: AB_2085327 (BioLegend Cat. No. 322807) AB_2261041 (BioLegend Cat. No. 322808)
Structure: Type I membrane receptor, forms functional heterodimeric complex with IL-7R to bind TSLP. Contains WSXWS motif required for proper protein folding and a box1 motif that is important for association with the JAKs.
Distribution: Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells. Also expressed in heart, skeletal, muscle, and kidney cells.
Function: TSLP binding to functional receptor activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Has been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells.
Interaction: Together with IL-7R binds TSLP
Cell Type: Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Reche PA, et al. 2001. J. Immunol. 167:336. 2. Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23. 3. Zhang W, et al. 2001. Biochem. Biophys. Res. Commun. 281:878. 4. Wang YH, et al. 2006. Immunity 24:827.
Gene ID: 64109
UniProt: View information about TSLPR on UniProt.org
Clone: 1B4
Regulatory Status: RUO
Other Names: Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype: Mouse IgG1, κ