Biolegend, 323020, PerCP/Cyanine5.5 anti-human CD15 (SSEA-1) Antibody, 100tests

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Martin R, et al. 2014. J Leukoc Biol. 96:151. PubMed Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed Friedman G, et al. 2016. Neuro Oncology. 18: 227 - 235. PubMed Li L, et al. 2015. J Am Soc Nephrol. 26: 2183-2197. PubMed Peranzoni E, et al. 2018. Proc Natl Acad Sci U S A. 115:E4041. PubMed Karakasheva TA, et al. 2018. JCI Insight. 3. PubMed Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed Galat Y, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0519-0. PubMed
RRID: AB_893259 (BioLegend Cat. No. 323019) AB_893257 (BioLegend Cat. No. 323020)
Structure: Poly-N-acetyllactosamine
Distribution: Neutrophils, eosinophils, monocytes
Function: Adhesion
Cell Type: Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID: NA
Clone: W6D3
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Lewis X, 3-FAL, 3-FL, LNFP III, Lex, SSEA-1, X-hapten
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.