Biolegend, 323022, Pacific Blue™ anti-human CD15 (SSEA-1) Antibody, 100tests

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 retinoblastoma cell line
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume. For µg size, the suggested use of this reagent for immunofluorescent staining is ≤1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Product Citations: Silvin A, et al. 2020. Cell. 182:1401. PubMed Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed Girard P, et al. 2022. Front Bioeng Biotechnol. 10:895735. PubMed Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed Køllgaard T, et al. 2015. PLoS One. 10: 0131934. PubMed Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed Iversen T, et al. 2014. Clin Cancer Res. 20:221. PubMed
RRID: AB_2105361 (BioLegend Cat. No. 323021) AB_2105359 (BioLegend Cat. No. 323024) AB_2105360 (BioLegend Cat. No. 323022)
Structure: Poly-N-acetyllactosamine
Distribution: Neutrophils, eosinophils, monocytes
Function: Adhesion
Cell Type: Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID: NA
Clone: W6D3
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Lewis X, 3-FAL, 3-FL, LNFP III, Lex, SSEA-1, X-hapten
Isotype: Mouse IgG1, κ