Biolegend, 323027, Brilliant Violet 510™ anti-human CD15 (SSEA-1) Antibody, 25tests

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed Scala S, et al. 2023. Nat Commun. 14:3068. PubMed Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed Smeenk L, et al. 2021. Cancer Discov. 11:2868. PubMed Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed Hofbauer D, et al. 2021. Immunity. 54(8):1772-1787.e9. PubMed Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed Yoshida R, et al. 2022. Cancer Res. :. PubMed Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed Chou DB, et al. 2022. Nat Commun. 13:6021. PubMed Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
RRID: AB_2561526 (BioLegend Cat. No. 323027) AB_2563400 (BioLegend Cat. No. 323028)
Structure: Poly-N-acetyllactosamine
Distribution: Neutrophils, eosinophils, monocytes
Function: Adhesion
Cell Type: Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID: NA
Clone: W6D3
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Lewis X, 3-FAL, 3-FL, LNFP III, Lex, SSEA-1, X-hapten
Isotype: Mouse IgG1, κ