Biolegend, 323305, PE anti-human CD109 Antibody, 25tests

The W7C5 monoclonal antibody recognizes human CD109 also known as the Gov platelet alloantigen. CD109 is a member of the alpha2-macroglobulin/complement gene family that is a GPI-linked cell surface antigen with a predicted molecular weight approximately 162 kD. CD109 contains a thioester motif characteristic of alpha-2-macroglobulin that undergoes autolytic cleavage under denaturing conditions. CD109 expression has been reported on CD34 + and CD34 - bone marrow stem cells, CD34 + cells in the fetal liver, CD34 + acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells. It is also widely expressed in the brain, uterus, heart, lung, and trachea of adults. The function of CD109 is largely unknown although it has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Giesert C, et al. In:Hematopoietic Stem Cells 2002:Genetics and function. Orlic D, et al. 2003. Ann. New York Acad. Sci. 996:227. Vogel W, et al. 2002. Haematologica 88:126.
Product Citations: Murugesan G, et al. 2022. Biomedicines. 10:. PubMed Bojic S, et al. 2018. Stem Cells. 36:1723. PubMed Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
RRID: AB_2073932 (BioLegend Cat. No. 323305)
Structure: Member of the alpha2-macroglobulin/complement gene family. GPI-linked cell surface antigen, predicted molecular weight approximately 162 kD.
Distribution: Expressed on CD34+ and CD34- bone marrow stem cells, CD34+ cells in the fetal liver, CD34+ acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells.
Function: Unknown; has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura
Cell Type: Hematopoietic stem and progenitors, Leukemia, Platelets, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Lin M, et al. 2002. Blood 99:1683. Schuh AC, et al. 2002. Blood 99:1692. Solomon KR, et al. 2004. Gene 327:171.
Gene ID: 135228
UniProt: View information about CD109 on UniProt.org
Clone: W7C5
Regulatory Status: RUO
Other Names: Gov platelet alloantigen
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.