Biolegend, 323508, PE/Cyanine7 anti-human CD140a (PDGFRα) Antibody, 100tests

The 16A1 monoclonal antibody recognizes human CD140a also known as the platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, and PDGFRα. CD140a is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140a contains three immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight of approximately 123 kD. CD140a is widely expressed on a variety of mesenchymal-derived cells and has been implicated in the development of some tumors including basal cell carcinoma and gastric stromal cell tumors. Binding of A-chain containing PDGF molecules as well as protease-activated PDGF-C molecules can stimulate cell proliferation. CD140a has been shown to interact with a number of proteins including CRK, Grb2, Grb14, SHP2, and others as integrin β3, caveolin-1, and nexin sorting molecules. The PDGFRα is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. The 16A1 antibody has been shown to be useful for flow cytometric detection of CD140a.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH 3T3 cells transfected with human PDGFRalpha
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Miyazaki S et al. In:Leukocyte Typing VI Kishimoto et al. Eds, Garland Publishing Inc, New York 1998 pp 3-20. Lottaz C, et al. 2010. Cancer Res. 70:2030. PubMed Ricono JM, et al. 2009. Am. J. Physiol. Renal Physiol. 296:F406. (IF) Guarnerio J, et al. 2015. Cancer Discov. 5:396. PubMed
Product Citations: Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed Lina IA, et al. 2022. Otolaryngol Head Neck Surg. 166:712. PubMed Boyd DF, et al. 2020. Nature. 587:466. PubMed Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed Agha O, et al. 2020. JOR Spine. 3:e1091. PubMed Kumar B, et al. 2022. Sci Adv. 8:eabh3375. PubMed Shi X, et al. 2022. Clin Transl Med. 12:e711. PubMed Yasuda T, et al. 2021. Cell Reports. 34(8):108779. PubMed Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
RRID: AB_2565596 (BioLegend Cat. No. 323507) AB_2565597 (BioLegend Cat. No. 323508)
Structure: Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family.
Distribution: Widely expressed on a variety of mesenchymal-derived cells.
Function: Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Knock-out studies have implicated an essential role for CD140a in kidney development. Has been implicated in basal cell carcinoma and gastric stromal cell tumors.
Interaction: Interacts with Crk, as well as a variety of adaptor molecules and signaling intermediates (Grb2, Grb14, SHP2, others). Has also been shown to associate with integrin β3, caveolin-1, and nexin sorting molecules
Ligand/Receptor: Binds to A-chain containing PDGF molecules and protease-activated PDGF-C molecules
Modification: Multiple tyrosine phosphorylation sites (Y720, Y731, Y742, Y754, Y762, Y767, Y768, Y988, Y993, Y1018)
Cell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Gronwald RG, et al. 1988. Proc. Natl. Acad. Sci. USA 85:3435. 2. Gilbertson DG, et al. 2001. J. Biol. Chem. 276:27406. 3. Seifert RA, et al. 1989. J. Biol. Chem. 264:8771. 4. Rupp E, et al. 1994. Eur. J. Biochem. 225:29.
Gene ID: 5156
UniProt: View information about CD140a on UniProt.org
Clone: 16A1
Regulatory Status: RUO
Other Names: Platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, PDGFRα, PDGFRa, PDGF receptor alpha
Isotype: Mouse IgG1, κ