Biolegend, 323607, APC anti-human CD140b (PDGFRβ) Antibody, 25tests

CD140b is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140b contains two immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 124 kD. CD140b is widely expressed on a variety of mesenchymal-derived cells and is preferentially expressed on some tumors such as medulloblastoma. Binding of B-chain containing PDGF molecules can stimulate cell proliferation. CD140b has been shown to interact with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Crk, Grb2, Grb4, RasGAP, SHP2, SHC1, others), and has also been shown to associate with integrin β3 and nexin sorting molecules. CD140b has been implicated in several disease states including atherogenesis and oncogenesis. The PDGFRβ is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH-3T3 cells transfected with human PDGFRbeta
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 18A2 monclonal antibody recognizes human CD140b also known as the platelet-derived growth factor receptor, beta polypeptide, PDGFR1, and PDGFRß. It has been shown to be useful for flow cytometric detection of CD140b.
Application References(PubMed link indicates BioLegend citation): Vogel W, et al. 2002. Haematologica 88:126. Arima, S., et al 2011. Development. 138:4763. PubMed.
Product Citations: Patterson AL, et al. 2020. Hum Reprod. 35:44:00. PubMed Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed Wong HY, et al. 2022. Nat Commun. 13:6036. PubMed Welsh JA, et al. 2022. Cell Rep Methods. 2:100136. PubMed Lin R, et al. 2014. Proc Natl Acad Sci U S A. 111:10137. PubMed Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
RRID: AB_2162787 (BioLegend Cat. No. 323608)
Structure: Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR1 and PD
Distribution: Widely expressed on a variety of mesenchymal-derived cells. Preferentially expressed in medulloblastoma.
Function: Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Has been implicated in atherogenesis and oncogenesis.
Interaction: Interacts with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Grb2, Grb4, RasGAP, SHP2, SHC1, Crk, others). Has also been shown to associate with integrin β3 and nexin sorting m
Ligand/Receptor: Binds to B-chain containing PDGF molecules as well as protease-activated PDGF-C
Modification: Multiple tyrosine phosphorylation sites (Y549, Y581, Y716, Y740, Y751, Y763, Y771, Y775, Y857, Y1009, Y1021)
Cell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Claesson-Welsh L, et al. 1988. Mol. Cell Biol. 8:3476. 2. Gronwald RG, et al. 1988. Proc. Natl. Acad. Sci. USA 85:3435. 3. Gilbertson DG, et al. 2001. J. Biol. Chem.276:27406. 4. Seifert RA, et al. 1989. J. Biol. Chem. 264:8771. 5. Kanakaraj P, et al. 1991. Biochemistry 30:1761.
Gene ID: 5159
UniProt: View information about CD140b on UniProt.org
Clone: 18A2
Regulatory Status: RUO
Other Names: Platelet-derived growth factor receptor, beta polypeptide, PDGFR1, PDGFRβ, PDGFRb, PDGF receptor beta
Isotype: Mouse IgG1, κ