Biolegend, 324006, PE anti-human CD318 (CDCP1) Antibody, 100tests

The CUB1 antibody recognizes CD318 also known as CDCP1, CUB domain containing protein 1, and SIMA135. CD318 is a transmembrane protein containing three CUB domains with a predicted molecular weight of approximately 93 kD. Alternately spliced isoforms have also been reported. CD318 is a marker for immature hematopoietic progenitor cell subsets and is also expressed in mesenchymal stem cells and neural progenitor cell populations. This marker is also widely distributed in a variety of cell types including keratinocytes, skeletal muscle, colon, kidney, lung, small intestine. CD318 is overexpressed in colon and lung tumors and expression level has been shown to correlate with metastatic ability of carcinomas. CD318 interacts with plasminogen, matriptase, and c-Src (phosphorylates residue Pyr734). The function of CD318 is not well understood although it is thought to be involved in early hematopoiesis. The CUB1 antibody has been shown to be useful for flow cytometry.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH-3T3 cells transfected with human CDCP1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Bühring HJ, et al. 2004. Stem Cells 22:334-343.
Product Citations: Miksiunas R, et al. 2022. Cells. 11:. PubMed Tomellini E, et al. 2020. Cell Reports. 28(4):1063-1073.e5.. PubMed
RRID: AB_2687086 (BioLegend Cat. No. 324017) AB_756056 (BioLegend Cat. No. 324006)
Structure: Transmembrane protein containing three CUB domains, predicted molecular weight approximately 93 kD. Alternately spliced isoforms have been reported
Distribution: Marker for immature hematopoietic progenitor cell subsets; also expressed in mesenchymal stem cells and neural progenitor cell populations. Widely distributed in a variety of cell types including keratinocytes, skeletal muscle, colon, kidney, lung, small
Function: Unknown, thought to be involved on early hematopoiesis
Interaction: Plasminogen, matriptase, c-Src
Modification: Phosphorylation (Y734) by c-Src; regulated by adhesion or plasmin in epithelial cells
Cell Type: Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Brown TA, et al. 2004. J. Biol. Chem. 279:14772. 2. Conze T, et al. 2003. Ann. N.Y. Acad. Sci. 996:222. 3. Hooper JD, et al. 2003. Oncogene 22:1783.
Gene ID: 64866
UniProt: View information about CD318 on UniProt.org
Clone: CUB1
Regulatory Status: RUO
Workshop: HLDA8
Other Names: CDCP1, CUB domain containing protein 1, SIMA135
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.