Biolegend, 324121, APC/Fire™ 750 anti-human CD324 (E-Cadherin) Antibody, 25tests

The 67A4 antibody recognizes human CD324 also known as E-cadherin, cadherin-1, and UVO. CD324, a member of the cadherin superfamily, is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region with a predicted molecular weight of approximately 100 kD. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the α E β 7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase 8, β-catenin, presenilin 1, casein kinase II , as well as other extracellular proteins including the EGF receptor. CD324 is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at reside D769 by caspase-3. The 67A4 antibody has been shown to be useful for flow cytometry.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: T-47D cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Armeanu S, et al. 1995. J. Cell Biol. 131:243. Bühring HJ, et al. 1996. Leukemia 10:106. Yauch RL, et al. 2005. Clin. Cancer Res. 11:8686. (WB) Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Ardehali R, et al. 2013. PNAS. 110:3405. PubMed Rasanen K, et al. 2013. Mol Cell Protecomics. 12:3778. PubMed Chaudhury A, et al. 2014. Nucleic Acids Res. 42:86. PubMed Milne P, et al. 2015. Blood. 125:470. PubMed
Product Citations: Gerli MFM, et al. 2024. Nat Med. 875:30. PubMed
RRID: AB_2687156 (BioLegend Cat. No. 324121) AB_2687157 (BioLegend Cat. No. 324122)
Structure: Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region. Predicted molecular weight approximately 100 kD.
Distribution: Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells
Function: Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cell, while the cytoplasmic domain is required for internalization.
Interaction: Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase 8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others
Ligand/Receptor: αEβ7 integrin
Modification: Phosphorylated on multiple residues (S857, S866, S870, S872), proteolytically cleaved at reside D769 by caspase-3.
Cell Type: Embryonic Stem Cells, Epithelial cells, Erythrocytes
Biology Area: Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Overduin M, et al. 1995. Science 267:386. 2. Boggon TJ, et al. 2002. Science 296:1303. 3. Berx G, et al. 1995. EMBO J. 14:6107. 4. Perl AK, et al. 1998. Nature 392:190.
Gene ID: 999
UniProt: View information about CD324 on UniProt.org
Clone: 67A4
Regulatory Status: RUO
Other Names: E-Cadherin, cadherin-1, and UVO
Isotype: Mouse IgG1, κ