Biolegend, 326008, PE anti-human CD84 Antibody, 100tests

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of (preferentially on CD45RO+) T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. The antibody CD84.1.21 is able to enhance CD3 induced IFN-γ production and partially block CD84-Ig binding to lymphocytes.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: 300.19 cell line transfected with the CD84 full-length DNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for the relevant formats) applications include: immunoprecipitation2, and enhancing CD3 induced IFN-? production1 and partially block CD84-Ig binding to lymphocytes1.
Application References(PubMed link indicates BioLegend citation): Martin M, et al. 2001. J. Immunol. 167:3668. Sayós J, et al. 2001. Blood 97:3867. Morra M, et al. 2001. EMBO 20:5840. Morra M, 2001. J. Biol. Chem. 276:36809. Romero X, et al. 2004. Tissue Antigens 64:132.
Product Citations: Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
RRID: AB_2074766 (BioLegend Cat. No. 326007) AB_2229003 (BioLegend Cat. No. 326008)
Structure: 64-82 kD glycoprotein, CD2 subset of Ig superfamily, SLAM family
Distribution: B cells, monocytes, thymocytes, platelets, subset of (preferentially on CD45RO+) T cells
Function: Homophilic adhesion, T cell activation
Ligand/Receptor: CD84
Cell Type: B cells, Monocytes, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. de la Fuente MA, et al. 1997. Blood 90 (6):2398. 2. Tangye SG, et al. 2002. Eur. J. Immunol. 32(6):1640. 3. Tangye SG, et al. 2003. J. Immunol. 171:2485. 4. Martin M, et al. 2001. J. Immunol. 167:3668.
Gene ID: 8832
UniProt: View information about CD84 on UniProt.org
Clone: CD84.1.21
Regulatory Status: RUO
Other Names: Ly9b, SLAMF5, GR6
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.