Biolegend, 327305, PE anti-human TNAP Antibody, 25tests

The W8B2 antibody reacts with an antigen, TNAP (Tissue non-specific alkaline phosphatase), that is selectively expressed on bone marrow mesenchymal stem cells, but not on placenta-derived mesenchymal stem cells. It was previously reported that the antibody recognized MSCA-1, mesenchymal stem cell antigen-1. Mesenchymal stem cells or MSCs were originally named as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Retinoblastoma cell line (Weri-RB-1 cells)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Battula VL, et al. 2009. Haematologica. 94:173.
Product Citations: Korody ML, et al. 2021. Stem Cells Dev. 30:177. PubMed Liang D, et al. 2022. Front Cell Dev Biol. 10:883314. PubMed Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
RRID: AB_940677 (BioLegend Cat. No. 327305) AB_940679 (BioLegend Cat. No. 327306)
Distribution: Bone marrow mesenchymal stem cells
Cell Type: Mesenchymal cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Battula VL, et al. 2007. Differentiation. 75:279.
Gene ID: 249
UniProt: View information about TNAP on UniProt.org
Clone: W8B2
Regulatory Status: RUO
Other Names: Tissue Non-specific Alkaline Phosphatase, mesenchymal stem cell antigen-1, MSC, W8B2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.