Biolegend, 327402, Purified anti-human SUSD2 Antibody, 100μg

The W5C5 antibody reacts with SUSD2 (sushi domain containing 2) which is expressed by bone marrow mesenchymal stem cells, vessel-surrounding smooth muscle cells, and a few neoplastic cell lines. SUSD2 is a recently identified type I transmembrane protein of 820 amino acids consisting of a large extracellular region containing a Somatomedin B (SMB), an Adhesion-associated domain in MUC4 and Other Proteins (AMOP), a von Willebrand factor (vWF), and a F1c Sushi domain. Mesenchymal stem cells (MSCs) were originally named bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells, and certain neuronal cells. MSCs reside in many cells and tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, liver, and lung. Besides their plasticity for tissue repair, MSCs also exhibit powerful immunosuppressive activity and play an important role in supporting hematopoiesis.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Retinoblastoma cell line (Weri-RB-1 cells).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence2.
Application References(PubMed link indicates BioLegend citation): Masuda H, et al. 2012. Cell Transplant. 21:2201. Sivasubramaniyan K, et al. 2013. Stem Cells Dev. 22:1944. PubMed (IF) Su K, et al. 2014. Acta Biomater. 10:5012. PubMed
Product Citations: Patterson AL, et al. 2020. Hum Reprod. 35:44:00. PubMed Rahimipour M, et al. 2021. Cell J. 23:154. PubMed van der Heide V, et al. 2022. Cell Rep. :110508. PubMed Sivasubramaniyan K, et al. 2013. Stem Cells Dev. 22:1944. PubMed Zhang S, et al. 2018. Stem Cell Res Ther. 9:48. PubMed
RRID: AB_940656 (BioLegend Cat. No. 327401)
Distribution: Bone marrow mesenchymal stem cells
Cell Type: Mesenchymal cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Buhring HJ, et al. 2007. Ann. N. Y. Acad. Sci. 1106:262. 2. Jackson L, et al. 2007. J. Postgrad. Med. 53:121. 3. Krampera M, et al. 2003. Blood 101:3722. 4. Ball LM, et al. 2007. Blood 110:2764.
Gene ID: 56241
UniProt: View information about SUSD2 on UniProt.org
Clone: W5C5
Regulatory Status: RUO
Other Names: Mesenchymal Stem Cells (MSC), Sushi domain containing 2 (SUSD2)
Isotype: Mouse IgG1, κ