Biolegend, 327505, PE anti-human SUSD2 Antibody, 25tests

The W3D5 antibody reacts with SUSD2 (sushi domain containing 2) which is expressed by bone marrow mesenchymal stem cells, vessel-surrounding smooth muscle cells, and a few neoplastic cell lines. SUSD2 is a recently identified type I transmembrane protein of 820 amino acids consisting of a large extracellular region containing a Somatomedin B (SMB), an Adhesion-associated domain in MUC4 and Other Proteins (AMOP), a von Willebrand factor (vWF), and a F1c Sushi domain. Mesenchymal stem cells (MSCs) were originally named bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells, and certain neuronal cells. MSCs reside in many cells and tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, liver, and lung. Besides their plasticity for tissue repair, MSCs also exhibit powerful immunosuppressive activity and play an important role in supporting hematopoiesis.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Retinoblastoma cell line (Weri-RB-1 cells).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Sivasubramaniyan K, et al. 2013. Stem Cells Dev. 22:1944. PubMed
Product Citations: Sivasubramaniyan K, et al. 2013. Stem Cells Dev. 22:1944. PubMed
RRID: AB_940629 (BioLegend Cat. No. 327506)
Distribution: Bone marrow mesenchymal stem cells
Cell Type: Mesenchymal cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Buhring HJ, et al. 2007. Ann. N. Y. Acad. Sci. 1106:262. 2. Jackson L, et al. 2007. J. Postgrad. Med. 53:121. 3. Krampera M, et al. 2003. Blood 101:3722. 4. Ball LM, et al. 2007. Blood 110:2764.
Gene ID: 56241
UniProt: View information about SUSD2 on UniProt.org
Clone: W3D5
Regulatory Status: RUO
Other Names: Mesenchymal Stem Cell antigen 2 (MSCA-2)
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.