Biolegend, 328102, Purified anti-human CD90 (Thy1) Antibody, 100μg

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34 + cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34 + CD90 + cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HEL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested CyTOF® - Verified IHC-F, ICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
Application References(PubMed link indicates BioLegend citation): Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP) Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity) Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope) Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
Product Citations: Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed Stice SL, et al. 2022. Environ Health Perspect. 130:117002. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed Floy ME, et al. 2022. STAR Protoc. 3:101275. PubMed Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed Azari S, et al. 2021. MBio. 12:e0184921. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed Lin J, et al. 2019. J Immunol Res. 2019:6929286. PubMed Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed Crawford LB, et al. 2021. J Virol. 95:. PubMed Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
RRID: AB_940390 (BioLegend Cat. No. 328101) AB_940393 (BioLegend Cat. No. 328102)
Structure: 25-35 kD glycoprotein, Ig superfamily
Distribution: Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines
Function: Regulate hematopoiesis and neural cell growth, cell adhesion
Ligand/Receptor: β3 integrin, β2 integrin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. McKenzie JL, et al. 1981. J. Immunol. 126:843. 2. Avalos AM, et al. 2002. Biol. Res. 35:231. 3. Wetzel A, et al. 2004. J. Immunol. 172:3850.
Gene ID: 7070
UniProt: View information about CD90 on UniProt.org
Clone: 5E10
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ