Biolegend, 328117, PerCP/Cyanine5.5 anti-human CD90 (Thy1) Antibody, 25tests

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34 + cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34 + CD90 + cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HEL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
Application References(PubMed link indicates BioLegend citation): Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP) Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity) Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope) Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
Product Citations: Wesely J, et al. 2020. Cell Reports. 31(9):107688. PubMed Marei H, et al. 2017. Sci Rep.. 10.1038/s41598-017-17882-1. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed Wang X, et al. 2021. Front Endocrinol (Lausanne). 12:666195. PubMed Kotini AG et al. 2017. Cell stem cell. 20(3):315-328 . PubMed Zhang Z, Shively J 2010. PLoS One. 5:e13985. PubMed Carter BZ, et al. 2019. Cancer Res. 79:1165. PubMed Croes M, et al. 2015. PLoS One. 10: 0132781. PubMed
RRID: AB_961312 (BioLegend Cat. No. 328117) AB_2303335 (BioLegend Cat. No. 328118)
Structure: 25-35 kD glycoprotein, Ig superfamily
Distribution: Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines
Function: Regulate hematopoiesis and neural cell growth, cell adhesion
Ligand/Receptor: β3 integrin, β2 integrin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. McKenzie JL, et al. 1981. J. Immunol. 126:843. 2. Avalos AM, et al. 2002. Biol. Res. 35:231. 3. Wetzel A, et al. 2004. J. Immunol. 172:3850.
Gene ID: 7070
UniProt: View information about CD90 on UniProt.org
Clone: 5E10
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.