Biolegend, 328201, Purified anti-human CD39 Antibody, 25μg

Human CD39 is an integral membrane protein with two transmembrane domains. It exists as a homotetramer. Expression of CD39 is found on activated lymphocytes, a subset of T cells and B cells, and dendritic cells with weak staining on monocytes and granulocytes. CD39 and CD73 have been found on regulatory T cells, specifically the effector/memory like T cells. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. CD39 is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.
25μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PHA activated human lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-P, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade3, immunofluorescence4, immunohistochemistry6, and spatial biology (IBEX)7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Aversa GG, et al. 1988. Transplant. P. 20:4952. Aversa GG, et al. 1989. Transplant. P. 21:34950. Borsellino G, et al. 2007. Blood. 110:1225. (Block) Stockl J, et al. 2001. J. Immunol. 167:2724. (IF) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed Brinkmann CR, et al. 2018. mSphere. 3:e00616. PubMed Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed Syrimi E, et al. 2021. iScience. 24:103215. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Henrick BM, et al. 2021. Cell. . PubMed Schwabenland M, et al. 2021. Immunity. . PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed NULL, et al. 2022. Cell. 185:916. PubMed Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
RRID: AB_940438 (BioLegend Cat. No. 328202)
Distribution: Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.
Cell Type: B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 953
UniProt: View information about CD39 on UniProt.org
Clone: A1
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: gp80, E-ATPDase, NTPDase-1, ecto-apyrase, Ec3.6.1.5
Isotype: Mouse IgG1, κ