Biolegend, 328213, Brilliant Violet 421™ anti-human CD39 Antibody, 25tests

Human CD39 is an integral membrane protein with two transmembrane domains. It exists as a homotetramer. Expression of CD39 is found on activated lymphocytes, a subset of T cells and B cells, and dendritic cells with weak staining on monocytes and granulocytes. CD39 and CD73 have been found on regulatory T cells, specifically the effector/memory like T cells. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. CD39 is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PHA activated human lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade3, immunofluorescence4, immunohistochemistry6, and spatial biology (IBEX)7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Aversa GG, et al. 1988. Transplant. P. 20:4952. Aversa GG, et al. 1989. Transplant. P. 21:34950. Borsellino G, et al. 2007. Blood. 110:1225. (Block) Stockl J, et al. 2001. J. Immunol. 167:2724. (IF) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: de Homdedeu M, et al. 2023. iScience. 26:106873. PubMed Röhl M, et al. 2021. Vaccines (Basel). 9:. PubMed Montané E, et al. 2017. PLoS One. 12(2):e0171294. PubMed Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed Kim DH, et al. 2021. eLife. 10:00. PubMed Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed Lam AJ, et al. 2021. Cell Reports. 36(5):109494. PubMed Zou F, et al. 2021. Mol Ther. 29:1794. PubMed Ellis-Connell AL, et al. 2018. J Virol. 92:e01748. PubMed Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
RRID: AB_10933084 (BioLegend Cat. No. 328213) AB_2564575 (BioLegend Cat. No. 328214)
Distribution: Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.
Cell Type: B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 953
UniProt: View information about CD39 on UniProt.org
Clone: A1
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: gp80, E-ATPDase, NTPDase-1, ecto-apyrase, Ec3.6.1.5
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.