Biolegend, 328321, PerCP/Cyanine5.5 anti-human CD49a Antibody, 25tests

CD49a is a 200 kD type I transmembrane glycoprotein also known as α 1 integrin, VLA-1 α chain, or Integrin α 1 . It associates with CD29 (β 1 integrin) to form VLA-1 complex, a collagen IV and alminin-1 receptor. It is expressed on activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CTL line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications include: immunoprecipitation1, immunohistochemical staining1 of acetone-fixed frozen tissue sections, and spatial biology (IBEX)3,4.
Application References(PubMed link indicates BioLegend citation): Hemler ME, et al. 1984. J.Immunol. 132:3011 Hemler ME, et al. 1985. J. Biol. Chem. 260:15246 Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID: AB_2876635 (BioLegend Cat. No. 328321) AB_2876635 (BioLegend Cat. No. 328322)
Structure: Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex
Distribution: Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells
Function: Adhesion, leukocyte migration
Ligand/Receptor: With integrin β1 (CD29) forms receptor for laminin-1 and collagen IV
Cell Type: Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells
Biology Area: Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122 Boiret N, et al. 2005. Exp. Hematol. 33:219
Gene ID: 3672
UniProt: View information about CD49a on UniProt.org
Clone: TS2/7
Regulatory Status: RUO
Other Names: α1 integrin, VLA-1 α chain, Integrin α1 chain, ITGA1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.