Biolegend, 328502, Purified anti-human CD102 (ICAM-2) Antibody, 100μg

CD102 is a 55 kD type I transmembrane glycoprotein, belongs to immunoglobulin superfamily, also known as Intercellular Adhesion Molecule-2, or ICAM-2. CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and in costimulation of immune response. CD102 is a counter receptor of LFA-1 (CD11a/CD18), DC-SIGN and MAC-1.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: ICAM-2 cDNA transfected COS cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications: immunoprecipitation(1), blocking the interaction between ICAM-2 and LFA-1(1), immunohistochemical staining(2) of frozen tissue sections
Application References(PubMed link indicates BioLegend citation): DeFougerolles AR,et al.1991.J. Exp.Med.174:253. Van Herpen CML,et al.2005.Clin. Cancer Res.11:1899
Product Citations: Kim N, et al. 2020. Cancers (Basel). 12:. PubMed
RRID: AB_1134245 (BioLegend Cat. No. 328502)
Structure: 55 kD type I transmembrane glycoprotein, Ig superfamily
Distribution: Vascular endothelial cells, lymphocytes, monocytes, platelets
Function: Adhesion, costimulation
Ligand/Receptor: LFA-1, DC-SIGN, MAC-1
Cell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1.Weber KSC, et al.2004.Inflammation.28:177. 2.Barclay A, et al.1997 The Leukoycte Antigen FactsbookAcademic Press. 3. Casasnovas JM, et al.1999.P. Natl. Acad. Sci. USA96:3017. 4. Diamonds MS, et al.1990. J. Cell Biol.111:3129.
Gene ID: 3384
UniProt: View information about CD102 on UniProt.org
Clone: CBR-IC2/2
Regulatory Status: RUO
Workshop: V BP363
Other Names: Intercellular Adhesion Molecule-2, ICAM-2
Isotype: Mouse IgG2a, κ