Biolegend, 328707, PE anti-human CD148 Antibody, 25tests

CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leucocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leucocytes. CD148 as a leucocyte activation marker and may be involved in the regulation of T cell activation. Leucocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PHA-stimulated human PBMC
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Tangye SG. 1998. J. Immunol. 161:3249. (FC)Tangye SG. 1998. J. Immunol. 161:3803.Tangye SG. 1998. J. Exp. Med. 188:1691.Autschbach F. 1999. Tissue Antigens 54:485.Lin J and Weiss A. 2003. J. Cell Biol. 162:673. (IF)Whiteford, JR., et al. 2011. Mol Cell Biol. 22:3609. PubMed.
Product Citations: Chappert P, et al. 2022. Immunity. 55:1872. PubMed
RRID: AB_2174826 (BioLegend Cat. No. 328708)
Distribution: Monocytes, T cells, B cells, NK cells, epithelial cells, fibrocytes, melanocytes and Schwann cells
Cell Type: Monocytes, T cells, B cells, NK cells, Epithelial cells, Fibroblasts, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 5796
UniProt: View information about CD148 on UniProt.org
Clone: A3
Regulatory Status: RUO
Other Names: HPTP-eta, DEP-1, p260
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.