Biolegend, 329502, Purified anti-human CD244 (2B4) Antibody, 100μg

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedWB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
Application References(PubMed link indicates BioLegend citation): Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397. Chuang SS, et al. 2000. Immunology. 100:378. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed Rossi LE, et al. 2012. J Leukoc Biol. 91:321. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Zhuang X, et al. 2019. Cancer Immunol Res. 0.94375. PubMed Hoffmann S, et al. 2011. J Immunol. 186:2757. PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed Kim N, et al. 2020. Cancers (Basel). 12:. PubMed Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
RRID: AB_1279194 (BioLegend Cat. No. 329502)
Structure: 38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution: NK, T subset, monocytes, basophils, and eosinophils
Function: Activation and inhibition of NK cell function
Ligand/Receptor: CD48
Cell Type: Basophils, Eosinophils, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Lee KM, et al. 2003. J. Immunol. 170:4881. 2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. 3. Messmer B, et al. 2006. J. Immunol. 176:4646. 4. Boles KS, et al. 2001. Immunol. Rev. 181:234.
Gene ID: 51744
UniProt: View information about CD244 on UniProt.org
Clone: C1.7
Regulatory Status: RUO
Workshop: VII 70350
Other Names: 2B4, SLAMF4
Isotype: Mouse IgG1, κ