Biolegend, 329534, Brilliant Violet 510™ anti-human CD244 (2B4) Antibody, 100tests

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
Application References(PubMed link indicates BioLegend citation): Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397. Chuang SS, et al. 2000. Immunology. 100:378. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed Rossi LE, et al. 2012. J Leukoc Biol. 91:321. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
RRID: AB_2814195 (BioLegend Cat. No. 329533) AB_2814196 (BioLegend Cat. No. 329534)
Structure: 38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution: NK, T subset, monocytes, basophils, and eosinophils
Function: Activation and inhibition of NK cell function
Ligand/Receptor: CD48
Cell Type: Basophils, Eosinophils, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Lee KM, et al. 2003. J. Immunol. 170:4881. 2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. 3. Messmer B, et al. 2006. J. Immunol. 176:4646. 4. Boles KS, et al. 2001. Immunol. Rev. 181:234.
Gene ID: 51744
UniProt: View information about CD244 on UniProt.org
Clone: C1.7
Regulatory Status: RUO
Workshop: VII 70350
Other Names: 2B4, SLAMF4
Isotype: Mouse IgG1, κ