Biolegend, 329601, Purified anti-human CD273 (B7-DC, PD-L2) Antibody, 25μg

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.
25μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PD-L2 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood.
Application References(PubMed link indicates BioLegend citation): Brown JA, et al. 2003. J. Immunol. 170:1257 Rodig N, et al. 2003. Eur. J. Immunol. 33:3117
Product Citations: Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Yonemitsu K, et al. 2022. Sci Rep. 12:12007. PubMed Komohara Y, et al. 2018. J Clin Exp Hematop. 58:152. PubMed Heesters BA, et al. 2021. J Exp Med. 218:. PubMed Hoffmann F, et al. 2020. Clin Epigenetics. 12:94. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Garcia de Moura R, et al. 2021. Front Immunol. 12:632667. PubMed Beetz O, et al. 2019. PLoS One. 14:e0220546. PubMed Enyindah-Asonye G, et al. 2019. Front Immunol. 10:511. PubMed
RRID: AB_1089010 (BioLegend Cat. No. 329602)
Structure: B7 Immunoglobulin superfamily, 25 kD
Distribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages
Function: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor: PD-1
Cell Type: Dendritic cells, Macrophages
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Shin T, et al. 2003. J. Exp. Med. 198:31. 2. Liu X, et al. 2003. J. Exp. Med. 197:1721. 3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
Gene ID: 80380
UniProt: View information about CD273 on UniProt.org
Clone: 24F.10C12
Regulatory Status: RUO
Other Names: B7-DC, PD-L2, PDL2, B7DC
Isotype: Mouse IgG2a, κ