Biolegend, 330107, APC anti-human CD209 (DC-SIGN) Antibody, 25tests

CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a criti­cal role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of human DC-SIGN
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1 and spatial biology (IBEX)2,3.
Application References(PubMed link indicates BioLegend citation): Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. (IHC-F) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Tang J, et al. 2023. Research (Wash D C). 6:0033. PubMed Zhang M, et al. 2022. Microbiol Spectr. 10:e0221221. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed Séguier S, et al. 2013. PLoS One. 8:70937. PubMed Fredriksson K, et al. 2014. J Immunol. 192:4497. PubMed Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed Paulikat AD, et al. 2022. J Innate Immun. 14:569. PubMed Ronaghan NJ, et al. 2022. PLoS One. 17:e0276013. PubMed
RRID: AB_1134055 (BioLegend Cat. No. 330107) AB_1134045 (BioLegend Cat. No. 330108)
Distribution: Dendritic cells
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.
Gene ID: 30835
UniProt: View information about CD209 on UniProt.org
Clone: 9E9A8
Regulatory Status: RUO
Other Names: Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin
Isotype: Mouse IgG2a, κ