Biolegend, 330805, PE anti-human MSC and NPC (W4A5) Antibody, 25tests

The W4A5 antibody was generated by immunization with Weri-RB-1 cells. It reacts with a subpopulation of mesenchymal stem cells and neural progenitor cells (NPCs). Mesenchymal stem cells or MSCs are originally name as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis. NPCs can give rise to astrocytes, neurons, and oligodendrocytes
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Retinoblastoma cell line (Weri-RB-1 cells)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_940642 (BioLegend Cat. No. 330806)
Distribution: Subpopulation of mesenchymal stem cells and neural progenitor cells
Cell Type: Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Buhring HJ, et al. 2007. Ann. N. Y. Acad. Sci. 1106:262. 2. Jackson L, et al. 2007 J. Postgrad. Med. 53:121. 3. Krampera M, et al. 2003 Blood 101:3722. 4. Ball LM, et al. 2007 Blood 110:2764
Gene ID: NA
UniProt: View information about MSC on UniProt.org
Clone: W4A5
Regulatory Status: RUO
Other Names: Mesenchymal Stem Cells and Neural Progenitor Cells
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.