Biolegend, 331013, PE/Cyanine7 anti-human CD101 (BB27) Antibody, 25tests

CD101 is a type I transmembrane glycoprotein, known as V7, and P126. It is a 120 kD homodimer, the member of EWI family within Ig superfamily. CD101 is found on monocytes, granulocytes, dendritic cells, a subpopulation of peripheral blood T cells and activated T cells. It has been reported that CD101 expressing Tregs posses higher inhibitory function. The biological function of CD101 is still not clear. But it has been found that BB27 antibody is able to inhibit T cell proliferative responses and this inhibitory function can be overcome by high doses of IL-2. Activation of CD101 on dendritic cells is able to induce IL-10 production.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Gouttefangeas C, et al. 1994. Int. Immunol. 6:423 Bouloc A, et al. 2000. Histopathology. 36:229
Product Citations: Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Hoang TN, et al. 2021. Cell. 184:460. PubMed Hoang TN, et al. 2020. bioRxiv. . PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
RRID: AB_2716108 (BioLegend Cat. No. 331013) AB_2716109 (BioLegend Cat. No. 331014)
Structure: Type I transmembrane glycoprotein, homodimer, Ig superfamily, 120 kD
Distribution: Monocytes, granulocytes, dendritic cells, T cell subset and activated T cells
Cell Type: Dendritic cells, Granulocytes, Monocytes, T cells
Biology Area: Immunology
Antigen References: 1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication 2. Bagot M, et al. 1997. Tissue Antigen 50:439
Gene ID: 9398
UniProt: View information about CD101 on UniProt.org
Clone: BB27
Regulatory Status: RUO
Workshop: V T040
Other Names: V7, P126
Isotype: Mouse IgG1, κ