Biolegend, 331120, PerCP/Cyanine5.5 anti-human/mouse CXCR7 Antibody, 100tests

CXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR). CXCR7 binds with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. Binding of ligand to CXCR7 induces proliferation and migration of immature neurons, glia and their precursors. CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. Surface expression of CXCR7 was also reported to be on tumor cells, activated endothelial cells, fetal liver cells, and other cell types.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR7 transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Soehnlein O, et al. 2013. EMBO Mol. Med. 5:471. (FC) PubMed Balabanian K, et al. 2012. J. Transl. Med. 10:251. (FC) PubMed
RRID: AB_2819960 (BioLegend Cat. No. 331119) AB_2860863 (BioLegend Cat. No. 331120)
Structure: G protein-coupled receptor super family
Distribution: Monocytes, B cells, T cells and mature dendritic cells
Ligand/Receptor: CXCL12/SDF-1 and CXCL11/I-TAC
Cell Sources: On a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells
Cell Type: B cells, Dendritic cells, Monocytes, T cells
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Burns JM, et al. 2006. J. Exp. Med. 203:2201. 2. Infantino S, et al. 2006. J. Immunol. 176:2197.
Regulation: Regulates the trafficking and activation of leukocytes
Gene ID: 1277857007
UniProt: View information about CXCR7 on UniProt.org
Clone: 8F11-M16
Regulatory Status: RUO
Other Names: Chemokine (C-X-C motif) receptor 7, RDC1, CMKOR1, GPR159
Isotype: Mouse IgG2b, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.