Biolegend, 331310, APC anti-human TCR Vγ9 Antibody, 100tests

The Vγ9 TCR is a variant of the TCR γ chain expressed on a subset of γ/δ T cells. Vγ9Vδ2 T lymphocytes, a major γ/δ T cell subset in humans, recognize phosphoantigens, certain tumor cells, and cells treated with aminobisphosphonates. This cell population displays cytolytic activity against various tumor cells. The γ/δ TCR is a heterodimeric TCR complex composed of covalently bound γ and δ chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains. The B3 antibody reacts specifically with human TCR Vγ9 as designated by the Lefranc/Foster nomenclature system. Human TCR Vγ9 is also known as TCR Vγ2 under the Strauss, Quertermous nomenclature system. Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts .
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Van Rhijn I, et al. 2003. Intl. Immunol. 15:373. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Poran A, et al. 2020. Cell Reports Medicine. 1(8):100141. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Ott PA, et al. 2020. Cell. 183(2):347-362.e24. PubMed de Bruin RC, et al. 2017. J Immunol. 198:308. PubMed Bruin R, et al. 2016. Clin Immunol. 169:128-38. PubMed Daniels J, et al. 2020. Nat Commun. 11:1806. PubMed Ogongo P, et al. 2020. J Clin Invest. 130:214. PubMed
RRID: AB_2057505 (BioLegend Cat. No. 331309) AB_2057504 (BioLegend Cat. No. 331310)
Structure: Ig superfamily member, TCR γ/δ with CD3 forms the CD3/TCR complex
Distribution: Expressed on majority of peripheral γ/δ T cells
Function: Antigen recognition, T cell activation
Ligand/Receptor: Peptide bound to MHC
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: Scotet E, et al. 2005. Immunity 22:71 Rincon-Orozco B, et al. 2005. J. Immunol. 175:2144
Gene ID: 6965
UniProt: View information about TCR Vgamma9 on UniProt.org
Clone: B3
Regulatory Status: RUO
Other Names: T cell receptor Vγ9, TCR Vγ2 (Strauss, Quertermous nomenclature), TRGV9, TCRGV2S1
Isotype: Mouse IgG1, κ