Biolegend, 331404, Biotin anti-human TCR Vδ2 Antibody, 100μg

The Vδ2 TCR is a variant of the TCR δ chain expressed on a subset of γ/δ T cells. Vγ9Vδ2 T lymphocytes, a major γ/δ T cell subset in humans, recognize phosphoantigens, certain tumor cells, and cells treated with aminobisphosphonates. This cell population displays cytolytic activity against various tumor cells. The γ/δ TCR is an heterodimeric TCR complex composed of covalently bound γ and δ chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains.
100μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Rojas RE, et al. 2005. J. Infect. Dis. 192:1806. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Ferry GM, et al. 2022. Front Immunol. 13:863155. PubMed Keller B, et al. 2016. J Exp Med. 213: 1185 - 1199. PubMed
RRID: AB_1089228 (BioLegend Cat. No. 331404)
Structure: Ig superfamily member, TCR γ/δ with CD3 forms the CD3/TCR complex
Distribution: Expressed on majority of peripheral γ/δT cells
Function: Antigen recognition, T cell activation
Ligand/Receptor: Peptide bound to MHC
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Scotet E, et al. 2005. Immunity 22:71. 2. Rincon-Orozco B, et al. 2005. J. Immunol. 175:2144.
Gene ID: 6964
UniProt: View information about TCR Vdelta2 on UniProt.org
Clone: B6
Regulatory Status: RUO
Other Names: T cell receptor V δ 2, TRDV2, TCRDV102S1
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.