Biolegend, 331606, PE anti-human CD276 (B7-H3) Antibody, 100tests

B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with mouse B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reported that monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo. Therefore, B7-H3 may be a negative regulator on T cell activation and function.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Watanabe T, et al. 2023. Polymers (Basel). 15:. PubMed Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed Fang C, et al. 2022. J Pathol. 256:310. PubMed Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed Brignole C, et al. 2021. J Exp Clin Cancer Res. 40:180. PubMed Huang C, et al. 2020. Theranostics. 10:10498. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Xie J, et al. 2021. FEBS Open Bio. 11:2977. PubMed Lavoie RR, et al. 2021. Cancers (Basel). 13:. PubMed Li H, et al. 2020. Front Oncol. 1.477083333. PubMed
RRID: AB_1279200 (BioLegend Cat. No. 331605) AB_1279197 (BioLegend Cat. No. 331606)
Structure: About 49.7 KD transmembrane protein characterized by extracellular IgV and IgC domains.
Distribution: B7-H3 is expressed on professional APCs, including B cells, dendritic cells, and macrophages. It is reported to express on a minor fraction of CD4+ and CD8+ cells.
Ligand/Receptor: The receptor has not been identified, but it seems to be expressed on activated T cells.
Bioactivity: B7-H3 is a negative regulator of T cell activation and IL-2 production.
Cell Type: Antigen-presenting cells, B cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Sun M, et al. 2002. J. Immunology. 168:6294 2. Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235
Gene ID: 80381
UniProt: View information about CD276 on UniProt.org
Clone: DCN.70
Regulatory Status: RUO
Other Names: B7RP2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.