Biolegend, 331808, PerCP/Cyanine5.5 anti-human CD319 (CRACC) Antibody, VO13

CD319 is a single-pass type l transmembrane glycoprotein, expressed on NK cells, subsets of mature dendritic cells, activated B cells, and cytotoxic lymphocytes, but not in promyelocytic, B or T cell lines. Expression is highest in the spleen, lymph nodes, and peripheral blood leukocytes, and lowest in bone marrow. Additionally, it is expressed in the small intestine, stomach, appendix, lung, and trachea. CD319 is tyrosine phosphorylated in activated NK cells and is associated with 19 and 39 kD proteins. CD319 has homology with the CD2 family of receptors within the Ig superfamily. Some of the CD2 members stimulate cytotoxicity through the CD319 associated protein.
VO13
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CRACC-Human IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Bouchon A, et al. 2001. J. Immunol. 167:551721. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002. Veillette A. 2006. Immunol. Rev. 214:2234.
Product Citations: Mondala PK, et al. 2021. Cell Stem Cell. 28(4):623-636.e9. PubMed
RRID: AB_2565234 (BioLegend Cat. No. 331813) AB_2565235 (BioLegend Cat. No. 331814)
Structure: Ig superfamily, 66 kD.
Distribution: NK cells, subsets of mature dendritic cells, activated B cells, cytotoxic lymphocytes but not in promyelocytic, B or T cell lines.
Function: Regulates T cell and NK cell functions.
Cell Type: B cells, Dendritic cells, Lymphocytes, NK cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Bouchon A, et al. 2001. J. Immunol. 167:551721. 2. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002. 3. Veillette A. 2006. Immunol. Rev. 214:2234.
Gene ID: 57823
UniProt: View information about CD319 on UniProt.org
Clone: 162.1
Regulatory Status: RUO
Other Names: CD2 like receptor activating cytotoxic cells, CD319, CS1, 19A24, SLAMF7
Isotype: Mouse IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.