Biolegend, 331946, Brilliant Violet 785™ anti-human CD335 (NKp46) Antibody, 100tests

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3 - CD56 + NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NKp46-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone 9E2 has been shown to block NK activation through NKp46.6
Application References(PubMed link indicates BioLegend citation): Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. Kalberer CP, et al. 2003. Blood 102:127. Chen Y, et al. 2007. J. Immunol. 179:2766. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Achdout H. et al. 2010. J. Virol. 84:3993.
Product Citations: Orta-Resendiz A, et al. 2023. STAR Protoc. 102734:4. PubMed Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
RRID: AB_2810508 (BioLegend Cat. No. 331945) AB_2810509 (BioLegend Cat. No. 331946)
Structure: Type I membrane glycoprotein (46 kD)
Distribution: Expressed on resting and activated NK cells
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147. 2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. 3. Sivori S. 1999. Eur. J. Immunol. 29:1656.
Gene ID: 9437
UniProt: View information about CD335 on UniProt.org
Clone: 9E2
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Mouse IgG1, κ