Biolegend, 332023, PerCP/Cyanine5.5 anti-human CD338 (ABCG2) Antibody, 25tests

CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘side population’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34.  In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells.  The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells.  The expression of ABCG2 appears to be highly conserved as it has been identified in various species.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone is listed under the following US Patent Numbers: 6,313,277; 7,541,437; 7,655,755; 7,741,455
Application References(PubMed link indicates BioLegend citation): Matalia H, 2012. Br J Ophthalmol. PubMed 2. Zhou S, et al. 2001. Nat. Med. 7:1028. (FC) Abbott BL, et al. 2002. Blood 100:4594. (FC) Huang FF, et al. 2104. PloS One. 9:88298. PubMed
RRID: AB_2814218 (BioLegend Cat. No. 332023) AB_2814219 (BioLegend Cat. No. 332024)
Distribution: primitive 'sidepopulation' (SP) stem cells, KDR+ human stem cells
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Neural Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Kim M, et al. 2002. Clin. Cancer Res. 8:228. 2. Scharenber CW, et al. 2002. Blood 99:50712. 3.Goodell MA, et al. 1997. Nat. Med. 3:133745. 4. Bunting KD. 2002. Stem Cells. 20:11.
Gene ID: 9429
UniProt: View information about CD338 on UniProt.org
Clone: 5D3
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: ABCG2, CDw338, Bcrp1, MXR
Isotype: Mouse IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.